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Ti2E/Ti2A/Ti2U
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Ti2E/Ti2A/Ti2U Ts2R-FL/Ts2R Ts2-FL/Ts2

Leading platform for advanced imaging.

The ECLIPSE Ti2 delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.

In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging.

Ti2-E

Ti2-A

Ti2-U

주요 특징들

Groundbreaking FOV
As research trends evolve towards large-scale, systems-level approaches, there is an increasing demand for faster data acquisition and higher throughput capabilities. Development of large-format camera sensors and improvements in the data processing capabilities of PCs have facilitated such research trends. The Ti2, with its unprecedented 25mm field of view, provides the next level of scalability, enabling researchers to truly maximize the utility of large-format detectors and future-proof their core imaging platform as camera technologies continue to develop at a rapid pace.
Bright illumination over a wide area
High-power LEDs deliver bright illumination across the Ti2's large field of view, ensuring clear, consistent results from demanding applications such as high-magnification DIC. Incorporation of a fly-eye lens design provides uniform illumination from edge to edge for quantitative high-speed imaging and seamless tiling of images in stitching applications.

High-power LED illuminator

Built-in fly-eye lens

A compact epi-fluorescence illuminator designed for large FOV imaging is equipped with a quartz fly-eye lens and provides high transmittance across a broad spectrum, including UV. Large diameter fluorescence filters with hard coatings deliver large FOV images with a high signal-to-noise ratio.

Large FOV epi-fl illuminator

Large diameter fluorescence filter cubes

Large diameter observation optics
The diameter of the observation light path has been enlarged in order to achieve a field number of 25 at the imaging port. The resulting large FOV is capable of capturing approximately double the area of conventional optics, enabling users to gain maximum performance from large-format sensors such as CMOS detectors.

Enlarged tube lens

Imaging port with large 25 field number

Objectives for large FOV imaging
Objectives with superior image flatness ensure high quality images from edge to edge. Utilizing the maximum potential of the OFN25 objective significantly accelerates data collection.
Cameras for large-volume data acquisition
The DS-Qi2 high-sensitivity monochrome camera and DS-Ri2 high-speed color camera are equipped with large 36.0 x 23.9 mm, 16.25 megapixel CMOS image sensors, enabling maximum performance with the Ti2's large 25mm FOV.
Unsurpassed Nikon optics
Nikon's high-precision CFI60 infinity optics, designed for use with a variety of sophisticated observation methods, are highly regarded by researchers for their superb optical performance and solid reliability.
Apodized phase contrast
Nikon's unique apodized phase contrast objectives with selective amplitude filters dramatically increase contrast and reduce halo artifacts to provide detailed high-definition images.

Apodizedphase plate is incorporated in APC objectives

BSC-1 cells captured with CFI S Plan Fluor ELWD ADM 40XC objective

External phase contrast
The motorized external phase-contrast system enables users to combine phase contrast with epi-fluorescence imaging without compromising fluorescent light transmission by bypassing the need to use phase-contrast objectives. For example, very high NA, liquid immersion objectives can be used for phase-contrast imaging. Using this external phase contrast system, users can easily combine phase contrast with other imaging modalities, including weak-fluorescence imaging such as TIRF and laser tweezer applications.

  • Eyepiece base unit with built-in phase ring
  • Phase ring
  • Objective lens without phase ring

Epi-fluorescence and external phase contrast images: PTK-1 cells labeled with GFP-alpha-tubulin captured with CFI Apochromat TIRF 100XC Oil objective. Photo courtesy of Alexey Khodjakov, Ph.D Research Scientist VI / Professor, Wadsworth Center

DIC (Differential Interference Contrast)
Nikon's highly-regarded DIC optics provide uniformly clear and detailed images with high resolution and contrast throughout the magnification range. DIC prisms are individually tailored for each objective lens to provide the highest-quality DIC images for every sample.

DIC prisms matched to individual objectives are mounted in the nosepiece

DIC and epi-fluorescence images: 25mm FOV image of neurons (DAPI, Alexa Fluor® 488, Rhodamine-Phalloidin), captured with CFI Plan Apochromat Lambda 60XC objective and DS-Qi2 camera Photo courtesy of Josh Rappoport, Nikon Imaging Center, Northwestern Univ.; Sample courtesy of S. Kemal, B. Wang, and R. Vassar, Northwestern Univ.

NAMC (Nikon Advanced Modulation Contrast)
This is a plastic-compatible, high-contrast imaging technique for unstained, transparent samples such as oocytes. NAMC provides pseudo-three-dimensional images with a shadow-cast appearance. The direction of contrast can be easily adjusted for each sample.

NAMC objective lenses contain rotatable modulators

NAMC image:
Mouse embryos, captured with CFI S Plan Fluor ELWD NAMC 20XC objective

Auto Correction Collar
Changes in sample thickness, cover glass thickness, refractive index distribution in the sample, and temperature can lead to spherical aberration and image deterioration. The highest quality objectives are often equipped with correction collars to compensate for these changes, and precise positioning of the collar is critical in achieving high resolution, high contrast images. This new auto correction collar utilizes a harmonic drive and automatic correction algorithm that enable users to easily achieve precise collar adjustment to achieve optimum objective performance every time.

Harmonic drive mechanism for high-precision control of correction collar movement

Super-resolution image (DNA PAINT):
CV-1 cells expressing α -tubulin (green) and TOMM-20 (magenta) captured with CFI Apochromat TIRF 100XC Oil objective.

Epi-fluorescence
The Lambda series objectives, utilizing Nikon's proprietary Nano Crystal Coat technology, are perfect for demanding, low-signal, multi-channel fluorescence imaging that requires high transmission and aberration correction over a wide wavelength range. Combined with new fluorescence filter cubes that offer improved fluorescence detection and stray light countermeasures such as the Noise Terminator, the Lambda series objectives demonstrate their power in weak signal observations such as single-molecule imaging and even luminescence-based applications.

Luminescence image:
HeLa cells expressing BRET-based calcium indicator protein, Nano-lantern (Ca2+).
Sample courtesy of Prof. Takeharu Nagai, The Institute of Scientific and Industrial Research, Osaka University

Volume Contrast New
Volume Contrast technique utilizes a series of label-free, brightfield images captured at various Z-depths to assemble a phase distribution image.

Volume Contrast renders cells easily identifiable as objects for automated counting and area analysis. As this method utilizes brightfield imaging, Volume Contrast enables in-line, non-destructive analysis of cells, suitable for a wide variety applications. Note. For Ti2-E only.

HeLa cells imaged with CFI S Plan Fluor ELWD 20XC

Features of Volume Contrast（VC)

Accurate identification of cells from label-free cultures for automated cell counting and area measurements.
Negligible Meniscus Effect on Cell Identification
Phase contrast images are negatively impacted at the edges of wells due to the meniscus effect. Volume Contrast bypasses this effect and enables cells at the edges of wells to be clearly identified, resulting in increased cell counts and improved statistics.
Focus perfected
Even the slightest change in temperature and vibrations in the imaging environment can greatly impact focus stability. The Ti2 eliminates focus drift using both static and dynamic measures to enable faithful visualization of the nanoscopicand microscopic world during long time-lapse experiments.
Mechanically redesigned for ultra-high stability
In order to improve the focusing stability, both Z-drive and PFS autofocusing mechanisms have been completely re-designed.

The new Z-focusing mechanism is smaller and positioned adjacent to the nosepiece to minimize vibrations. It remains adjacent to the nosepiece even in an expanded (staged-up) configuration, ensuring stability for all applications.

① High stability Z-focusing mechanism remains adjacent to the nosepiece even in expanded configurations

The detector portion of the Perfect Focus System (PFS) has been detached from the nosepiece in order to reduce mechanical load on the objective nosepiece. This new design also minimizes heat transfer, which contributes to a more stable imaging environment. Towards this end, the power consumption of the Z-drive motor has also been reduced. Combined, these mechanical redesigns result in an ultra-stable imaging platform, perfectly suited for single-molecule imaging and super-resolution applications.

High stability Z-focusing mechanism remains adjacent to the nosepiece even in expanded configurations.
① PFS nosepiece
② PFS Measuring Unit

Real time focus correction with the PFS: Simply perfect
The Perfect Focus System (PFS) automatically corrects focus drift caused by temperature changes and mechanical vibrations, which can be caused by a variety of factors including the addition of reagents to the sample and multi-position imaging.

The PFS maintains focus by detecting and tracking the position of the coverslip surface in real time. Unique optical offset technology allows users to easily maintain focus at a desired position offset from the cover slip surface. The PFS automatically and continuously maintains focus by means of a built-in linear encoder and high speed feedback mechanism, providing highly reliable images even during long-term, complex imaging tasks.

PFS is compatible with a wide range of applications, from routine experiments involving plastic culture dishes to single-molecule imaging and multi-photon imaging. It is also compatible with a wide range of wavelengths, from ultraviolet to infrared, meaning it can be used for multi-photon and optical tweezer applications.
Water Immersion Dispenser
The performance of long-term imaging using the PFS together with water immersion objectives can be increased by using the new Water Immersion Dispenser. The Water Immersion Dispenser automatically applies the appropriate amount of pure water to the tip of an objective, preventing the immersion liquid from drying out and overflowing during experiments. It is compatible with all types of water immersion objectives and helps to stably provide high-resolution, high-contrast and aberration-corrected time-lapse images over long periods of time.
Compatible objectives
  • CFI Apochromat LWD Lambda S 20XC WI
  • CFI Apochromat Lambda S 40XC WI
  • CFI Apochromat LWD Lambda S 40XC WI
  • CFI Plan Apochromat VC 60XC WI
  • CFI Plan Apochromat IR 60XC WI
  • CFI SR Plan Apochromat IR 60XC WI
  • CFI SR Plan Apochromat IR 60XAC WI
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